![]() We use the eLONGase enzyme mix in a combined master mix format (i.e., Taq polymerase, eLONGase buffers, and dNTPs combined - excluding PCR primers and DNA) and stored frozen at -20° C in a PCR-ready 96-well plate.Īfter thawing this PCR-ready plate, gene specific forward and reverse primers are added along with target DNA. We have found the GibcoBRL Life Technologies eLONGase PCR reaction kit to work on approximately 80% of our ![]() Specific reactions, and allows for most PCR reactions to be done under a standard set of conditions. ![]() (Perkin-Elmer)) to identify a kit which produces simple, robust, and very We have tested numerous PCR reagent kits from different vendors (e.g., AmpliTaq
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